Identical genes for trimethoprim‐resistant dihydrofolate reductase from Staphylococcus aureus in Australia and Central Europe

FEBS Letters

Then, Rudolf L.¹  Ott, Michael¹  Burdeska, Alexander¹  Bannwarth, Willi¹ 

[1] Pharmaceutical Research Department, F. Hoffmann-La Roche Ltd., CH-4002 Basel, Switzerland

关键词: Trimethoprim resistance;    Dihydrofolate reductase;    Staphylococcus aureus;    Transposon Tn4OO3;    Pc;    penicillin G;    Cd;    cadmium;    Cm;    chloramphenicol;    Ap;    ampicillin;    MTX;    methotrexate;    DHFR;    dihydrofolate;    reductase;    Tp;    trimethoprim;    TS;    thymidylate synthetase;    ORF;    open reading frame;   

DOI  :  10.1016/0014-5793(90)81529-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nucleotide sequence of a 1.25 kb BglII/EcoRI fragment from the 34 kb trimethoprim (Tp)-resistant plasmid pABU1 of Staphylococcus aureus 157/4696, isolated in Zürich, was determined. It contained the entire Tp-resistant dihydrofolate reductase gene, 197 bp of the thymidylate synthetase, 395 bp of a truncated gene and 111 bp of IS257R1. With the exception of one single base pair at position 862 the sequence of the whole fragment was identical to nucleotides 1633 to 2885 of the Tp-resistant transposon Tn4OO3 in plasmid pSK 1 from an Australian S. aureus isolate. This suggests the worldwide dissemination of Tn4OO3 in multiresistant Staphylococci.

【 授权许可】

Unknown