Prediction of domain organisation and secondary structure of thyroid peroxidase, a human autoantigen involved in destructive thyroiditis

FEBS Letters

Sutton, Brian J.³  Odell, Eddy²  McGregor, Alan M.⁴  Saldanha, Jose W.¹  Mahadevan, Daruka⁴  Barton, Geoffrey J.¹  Banga, J.Paul⁴ 

[1] Imperial Cancer Research Fund Laboratories, Biomedical Computing Unit, PO Box 123, Lincoln's Inn Fields, London WC2A 3PX UK;Department of Oral Medicine and Pathology, United Medical and Dental Schools, Guy's Hospital, Floor 28, Guys Tower, London Bridge, London SE1 9RT, UK;Division of Biomolecular Sciences, Biophysics Section, King's College London, 26-29 Drury Lane, London WC2B 5RL UK;Department of Medicine, King's College School of Medicine, Denmark Hill, London SE5 8RX UK

关键词: Thyroid peroxidase;    Autoantigen;    Myeloperoxidase;    Circular dichroic spectroscopy;    Secondary structure prediction;   

DOI  :  10.1016/0014-5793(90)81524-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Organ specific autoimmune diseases are relatively common immunological disorders in man which include thyroid autoimmune disease, insulin-dependent diabetes mellitus and myasthenia gravis. The target autoantigens in some of these diseases have recently been characterised. In thyroid autoimmune disease this includes the key enzyme, thyroid peroxidase (TPO), which is involved in the generation of thyroid hormone. Structural knowledge about autoantigens such as thyroid peroxidase will allow a greater understanding of the interaction between autoantigens and the aberrant immune response, and facilitate the development of strategies for antigen-specific therapeutic manipulation. We report here a prediction of the secondary structure of thyroid peroxidase, together with the results of circular dichroic spectroscopy of a homologous purified enzyme. A combination of 3 secondary structure prediction programs has been used, following multiple sequence alignment, and TPO has been found to consist mainly of α-helical conformation, with little β-sheet present. This structure prediction, together with knowledge of the exon-intron boundaries allows a model for the domain organisation of the TPO molecule to be proposed.

【 授权许可】

Unknown