The cell adhesion molecule Cell‐CAM 105 is an ecto‐ATPase and a member of the immunoglobulin superfamily

FEBS Letters

Lazrek, Mohamed B.S.¹  Öbrink, Björn¹  Aurivillius, Magnus¹  Bock, Elisabeth²  Hansen, Ole C.² 

[1] Department of Medical Cell Biology, Medical Nobel Institute, Karolinska Institutet, Box 60400, S-104 01 Stockholm, Sweden;The Protein Laboratory, University of Copenhagen, Sigurdsgade 34, 2200 Copenhagen, Denmark

关键词: Cell adhesion;    Immunoglobulin superfamily;    ATP;   

DOI  :  10.1016/0014-5793(90)80264-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cell-CAM 105 (C-CAM), a cell adhesion molecule in rat hepatocytes, was digested with trypsin, and peptides were isolated and sequenced by Edman degradation. The sequences of 4 peptides agreed with different regions of rat liver ecto-ATPase. Detailed biochemical analyses confirmed the identity between C-CAM and the ecto-ATPase. C-CAM/ecto-ATPase is a transmembrane protein having 4 immunoglobulin-like domains in the extracellular portion, demonstrating membership of the immunoglobulin superfamily. The ATPase activity suggests that ATP might influence cell adhesion, which would explain the inhibitory effect of exogenously added ATP on adhesion of several cell types.

【 授权许可】

Unknown