期刊论文详细信息
FEBS Letters
The GC‐selective ligand mithramycin alters the structure of (AT)n sequences flanking its binding sites
Fox, Keith R.1  Cons, Benjamin M.G.1 
[1] Dept. Physiology & Pharmacology, University of Southampton, Bassett Crescent East, Southampton S09 3TU, UK
关键词: Mithramycin;    DNase I;    Footprinting;    DNA structure;    (AT)n;   
DOI  :  10.1016/0014-5793(90)80775-E
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

DNA fragments containing (AT)n inserts cloned adjacent to putative mithramycin binding sites have been examined by footprinting experiments using a variety of nucleases in the presence of the drug. The results demonstrate that mithramycin induces a DNA structural change which renders adjacent (AT)n sequences sensitive to attack by DNase II. Significant changes are also revealed with DNase I and micrococcal nuclease. The results are consistent with a model in which mithramycin opens the DNA minor groove changing it to a structure which is locally more like A-DNA.

【 授权许可】

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