The GC‐selective ligand mithramycin alters the structure of (AT)n sequences flanking its binding sites

FEBS Letters

Fox, Keith R.¹  Cons, Benjamin M.G.¹ 

[1] Dept. Physiology & Pharmacology, University of Southampton, Bassett Crescent East, Southampton S09 3TU, UK

关键词: Mithramycin;    DNase I;    Footprinting;    DNA structure;    (AT)n;   

DOI  :  10.1016/0014-5793(90)80775-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA fragments containing (AT)_n inserts cloned adjacent to putative mithramycin binding sites have been examined by footprinting experiments using a variety of nucleases in the presence of the drug. The results demonstrate that mithramycin induces a DNA structural change which renders adjacent (AT)_n sequences sensitive to attack by DNase II. Significant changes are also revealed with DNase I and micrococcal nuclease. The results are consistent with a model in which mithramycin opens the DNA minor groove changing it to a structure which is locally more like A-DNA.

【 授权许可】

Unknown