DNA methylation in Trypanosoma cruzi

FEBS Letters

Galanti, Norbel¹  Rojas, M.Véronica¹ 

[1] Department of Cell Biology and Genetics, School of Medicine, University of Chile, Casilla 70061, Santiago 7, Chile

关键词: DNA methylation;    5-Methylcytosine;    Base composition;    Trypanosoma cruzi;    m5Cyt;    5-methylcytosine;    m6Ade;    N6-methyladenine;    HPLC;    high-pressure liquid chromatography;    PBS;    phosphatebuffered saline;    EDTA;    ethylenediamine-tetraacetic acid;    NDNA;    nuclear DNA;    kDNA;    kinetoplast DNA;    SDS;    sodium dodecyl sulphate;    5-aza-C;    5-azacytidine;   

DOI  :  10.1016/0014-5793(90)80717-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5-methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence -CCGG- is not methylated. Probably T. cruzi DNA also contains N⁶-methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.

【 授权许可】

Unknown