Factor Xa cleavage of fusion proteins

FEBS Letters

Wearne, Steven J.¹ 

[1] MRC Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK

关键词: Fusion protein cleavage;    Factor Xa;    Protein modification;    THPA;    3;    4;    5;    6-tetrahydrophthalic anhydride;    pMb;    porcine myoglobin;    P37Y;    37Pro→Tyr;   

DOI  :  10.1016/0014-5793(90)80696-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A method is described for the elimination of non-specific cleavage of fusion proteins by factor Xa. Putative non-specific cleavage sites C-terminal to lysyl residues are blocked by reversible acylation by 3,4,5,6-tetrahydrophthalic anhydride prior to cleavage. After cleavage, the acyi groups are removed quantitatively by exposure to slightly acidic conditions. This method employs no harsh reagents or conditions, and may be generally applicable to factor Xa cleavage of fusion proteins.

【 授权许可】

Unknown