Antioxidant effects of Dendropanax morbifera Léveille extract in the hippocampus of mercury-exposed rats

BMC Complementary and Alternative Medicine

In Koo Hwang²  Yeo Sung Yoon²  Seung Myung Moon³  Jung Hoon Choi¹  Dong-Woo Kim⁴  Jong Whi Kim²  Hyo Young Jung²  Dae Young Yoo²  Dae Won Kim⁵  Woosuk Kim² 

[1] Department of Anatomy, College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, South Korea;Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul 151-742, South Korea;Department of Neurosurgery, Dongtan Sacred Heart Hospital, College of Medicine, Hallym University, Hwaseong 445-170, South Korea;Central Research Center, Egreen Co. Ltd, Seongnam 463-862, South Korea;Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Kangneung-Wonju National University, Gangneung 210-702, South Korea

关键词: Protein modification;    Reactive oxygen species;    Hippocampus;    Mercury;    Dendropanax morbifera extract;   

Others  :  1221181
DOI  :  10.1186/s12906-015-0786-1

 received in 2015-03-19, accepted in 2015-07-17,  发布年份 2015

【 摘 要 】

Background

Dendropanax morbifera Léveille has been employed for the treatment of infectious diseases using folk medicine. In this study, we evaluated the antioxidant effects of a leaf extract of Dendropanax morbifera Léveille in the hippocampus of mercury-exposed rats.

Methods

Seven-week-old Sprague–Dawley rats received a daily intraperitoneal injection of 5 μg/kg dimethylmercury and/or oral Dendropanax morbifera Léveille leaf extract (100 mg/kg) for 4 weeks. Animals were sacrificed 2 h after the last dimethylmercury and/or leaf extract treatment. Mercury levels were measured in homogenates of hippocampal tissue, a brain region that is vulnerable to mercury toxicity. In addition, we measured reactive oxygen species production, lipid peroxidation levels, and antioxidant levels in these hippocampal homogenates.

Results

Treatment with Dendropanax morbifera Léveille leaf extract significantly reduced mercury levels in hippocampal homogenates and attenuated the dimethylmercury-induced increase in the production of reactive oxygen species and formation of malondialdehyde. In addition, this leaf extract treatment significantly reversed the dimethylmercury-induced reduction in the hippocampal activities of Cu, Zn-superoxide dismutase, catalase, glutathione peroxidase, and glutathione-S-transferase.

Conclusion

These results suggest that a leaf extract of Dendropanax morbifera Léveille had strong antioxidant effects in the hippocampus of mercury-exposed rats.

【 授权许可】

   
2015 Kim et al.

【 图 表 】

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