Sequence location of a putative transglutaminase cross‐linking site in human vitronectin

FEBS Letters

Skorstengaard, Karna¹  Højrup, Peter³  Halkier, Torben¹  Mosher, Deane² 

[1] Department of Molecular Biology, University of Aarhus, 8000 Aarhus C, Denmark;Department of Physiological Chemistry, University of Wisconsin, Madison, WI 53706, USA;Department of Molecular Biology, University of Odense, 5230 Odense M, Denmark

关键词: Vitronectin;    Serum spreading factor;    S-protein;    Transglutaminase;    Factor XIIIa;    Cross-linking;    VN;    vitronectin;    PTH;    phenylthiohydantoin;    HPLC;    high-performance liquid chromatography;    PDMS;    plasma desorption mass-spectrometry;    FXIII;    plasma factor XIII;    putrescine;    1;    4-diamino butane;    S. aureus protease;    Staphyloccocus aureus V8 protease;    a.m.u.;    atomic mass units;   

DOI  :  10.1016/0014-5793(90)80208-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We attempted to locate the glutamine residue in human vitronectin, susceptible to cross-linking by transglutaminases. Vitronectin was incubated with ¹⁴C-labelled putrescine and plasma factor XIIIa and, after reduction and alkylation, the vitronectin was digested with trypsin. HPLC of the digest followed by scintillation counting revealed one major and two minor radioactivity labelled peaks. Sub-digestion with Staphylococcus aureus protease, sequence analysis and mass-spectrometry of the resulting peptides demonstrated that Gln-93 of vitronectin had incorporated putrescine. Additionally, Gln-73, Gln-84 and Gln-86 were found to be minor sites for incorporation.

【 授权许可】

Unknown