Inhibition of microtubule assembly in vitro by anti‐tubulin monoclonal antibodies

FEBS Letters

Dráber, P.²  Vater, W.²  Unger, E.²  Böhm, K.J.²  Kuklova, E.¹ 

[1] Institute of Molecular Genetics, Czechoslovak Academy of Sciences, 142 20 Prague 4, Czechoslovakia;Central Institute of Microbiology and Experimental Therapy, Academy of Sciences of the GDR, Jena, GDR

关键词: Tubulin domain;    Microtubule protein;    Microtubule assembly;    Monoclonal antibody;    (m)Ab(s);    (monoclonal)antibody(ies);    MES;    2-(N-morpholino)ethanesulphonic acid;    MTP;    microtubule protein;    SDS-PAGE;    polyacrylamide gel electrophoresis with sodium dodecylsulphate;    SwAM;    swine antibody against mouse immunoglobulin;   

DOI  :  10.1016/0014-5793(90)80191-K

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Five monoclonal antibodies against N-terminal domains of α- or β-tubulin were tested for their ability to interfere with the in vitro formation of microtubules. Although all the antibodies exhibited similar association constants for immobilized tubulin, they differed in their inhibitory effect on microtubule assembly. For the most potent antibody, TU-13, the antibody/tubulin molar ratio of about 1:320 was sufficient for a 50% inhibition. The data indicate that the surface regions of N-terminal domains of tubulin are involved in the formation of microtubules.

【 授权许可】

Unknown