Comparison of mitochondrial cationic channels in wild‐type and porin‐deficient mutant yeast

FEBS Letters

Fèvre, Florence³  Thieffry, Michel³  Chich, Jean-François¹  Henry, Jean-Pierre¹  Lauquin, Guy J.M.² 

[1] Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France;Institut de Biochimie Cellulaire et Neurochimie, CNRS, 1 rue Camille Saint Saëns, 33077 Bordeaux Cedex, France;Laboratoire de Neurobiologie Cellulaire et Moléculaire, CNRS, 91198 Gif sur Yvette Cedex, France

关键词: Ionic channels;    Mitochondria;    Porin-deficient mutant;    (Yeast);    VDAC;    voltage-dependent anion channel;    TEA;    tetraethylammonium;   

DOI  :  10.1016/0014-5793(90)80189-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bilayers were formed at the tip of microelectrodes from a suspension of proteoliposomes derived from wild-type and porin-deficient mutant yeast mitochondria. In both preparations, identical cationic channels of large conductance were recorded. This result rules out any relationship between this channel and the outer membrane voltage-dependent anion channel, the activity of which is carried by porin. The ionic selectivity and the voltage-dependence of the yeast cationic channel suggest that it is related to that recently described in mammalian mitochondria. This hypothesis is further supported by the fact that both channels are blocked by a mitochondrial addressing peptide.

【 授权许可】

Unknown