Suppression of nuclear ADP‐ribosyltransferase activity in Ehrlich ascites tumor cells by 5‐azacytidine

FEBS Letters

Nothacker, Hans-Peter¹  Hoshino, Jiro¹  Kröger, Hans¹  Schalge, Sigrid¹ 

[1] Robert Koch-Institut, Nordufer 20, D-1000 Berlin 65, FRG

关键词: ADP-ribosylation reaction;    in vitro;    5-azacytidine-modified DNA;    Histone requirement;    (Ehrlich ascites tumor cell);    ADPRT;    ADP-ribosyltransferase;    5-ACR;    5-azacytidine;    EAT;    Ehrlich ascites tumor;   

DOI  :  10.1016/0014-5793(90)80154-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Exposure of Ehrlich ascites tumor cells to 5-azacytidine for 5 h resulted in a partial loss of ability of DNA to stimulate ADP-ribosyltransferase activity, as assessed in a reconstituted in vitro enzyme system consisting of purified calf thymus enzyme, calf thymus whole histone and DNA isolated from the cells. The degree of suppression in vitro varied depending on the amount of histone and DNA added and it reached a maximum with a value of 83% and 62% of control for DNAs from cells exposed to 10 μM and 30 μM 5-azacytidine, respectively, at a histone/DNA mass ratio of 0.4. In the absence of histone (conditions of auto-ADP-ribosylation of the enzyme), no suppression was detectable.

【 授权许可】

Unknown