Mapping of a dominant immunogenic region of synaptophysin, a major membrane protein of synaptic vesicles

FEBS Letters

Betz, Heinrich¹  Knaus, Petra¹ 

[1] Zentrum für Molekulare Biologie, Universität Heidelberg, Im Neuenheimer Feld 282, 6900 Heidelberg, FRG

关键词: Synaptophysin;    Synaptic vesicle;    Antigenic epitope;    Site-directed mutagenesis;    (Rat brain);    mAb;    monoclonal antibody;    SCY;    synaptophysin cytoplasmic tail;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(90)80591-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synaptophysin is a major integral membrane protein of synaptic vesicles. Its transmembrane topology deduced from the cDNA sequence predicts 4 transmembrane regions and a carboxy-terminal cytoplasmic tail containing a characteristic pentapeptide repeat structure. The monoclonal antibody (mAb), SY38, binds to a cytoplasmic domain of synaptophysin. By using fusion proteins corresponding to truncated forms of the cytoplasmic tail, its epitope was located to a flexible segment in the center of the repeat structure. Four other mAbs (c7.1, c7.2, c7.3, c7.4) share the same epitope, which thus emerges as the major immunogenic region of this membrane protein.

【 授权许可】

Unknown