Fractionation of synaptophysin‐containing vesicles from rat brain and cultured PC12 pheochromocytoma cells

FEBS Letters

Wiedenmann, Bertram²  Knierim, Martin²  Becker, Cord-Michael¹  Rehm, Hubert¹ 

[1] Center for Molecular Biology, University of Heidelberg, D-6900 Heidelberg, FRG;Department of Internal Medicine, Ludolf-Krehl-Klinik, University of Heidelberg, D-6900 Heidelberg, FRG

关键词: Synaptophysin;    Synaptic vesicle;    Dense core vesicle;    Dot immunoassay;    BSA;    bovine serum albumin;    NC;    nitrocellulose;    PAGE;    polyacrylamide gel electrophoresis;    SCV;    synaptophysin-containing vesicles;   

DOI  :  10.1016/0014-5793(88)80342-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synaptophysin is a transmembrane glycoprotein of neuroendocrine vesicles. Its content and distribution in subcellular fractions from cultured PC12 cells, rat brain and bovine adrenal medulla were determined by a sensitive dot immunoassay. Synaptophysin-containing fractions appeared as monodispersed populations similar to synaptic vesicles in density and size distribution. Membranes from synaptic vesicles contained ≈ 100-times more synaptophysin than chromaffin granules. In conclusion, synaptophysin is located almost exclusively in vesicles of brain and PC12 cells which are distinct from dense core granules.

【 授权许可】

Unknown