Potentiation of angiotensin II‐stimulated phosphoinositide hydrolysis, calcium mobilization and contraction of renal mesangial cells upon down‐regulation of protein kinase C

FEBS Letters

Pfeilschifter, Josef¹  Whitebread, Steven¹  de Gasparo, Marc¹  Ochsner, Martin¹  Fandrey, Joachim² 

[1] Ciba-Geigy Ltd, Pharmaceuticals Division and Central Function Research, CH-4002 Basel, Switzerland;Institut für Physiologie, Medizinische Universität zu Lübeck, Ratzeburger Allee 160, D-2400 Lübeck, FRG

关键词: Angiotensin II;    Phosphoinositide;    Ca2+;    Protein kinase C;    Contraction;    Mesangial cell;   

DOI  :  10.1016/0014-5793(90)80578-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Long-term pretreatment of rat mesangial cells with 12-O-tetradecanoylphorbol 13-acetate (TPA) down-regulated protein kinase C activity and potentiated the angiotensin II-induced inositol trisphosphate (InsP₃) formation. This increased response to angiotensin II occurred without a significant change in the receptor number or K _d value of angiotensin II binding to the cells. The biologically inactive phorbol ester 4α-phorbol 12,13-di-decanoate was without effect on angiotensin II-stimulated InsP₃ generation. Long-term pretreatment with TPA also increased the angiotensin II-induced mobilization of Ca²⁺ and the subsequent contraction of mesangial cells.

【 授权许可】

Unknown