Two phases of inositol polyphosphate and diacylglycerol production at fertilisation

FEBS Letters

Whitaker, Michael¹  Ciapa, Brigitte¹ 

[1] Department of Physiology, University College London, Gower Street, London WC1E 6BT, England

关键词: Inositol trisphosphate Diacylglycerol Phosphoinositide Ca2+ release Protein kinase C Fertilization;   

DOI  :  10.1016/0014-5793(86)80191-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

[³H]Inositol and [³H]arachidonic acid were used to label polyphosphoinositide phospholipids in sea urchin eggs. Both [³H]inositol polyphosphate (InsP₃) and [³H]diacylglycerol (DAG) increase at fertilisation. An early increase in InsP₃ occurs as the sperm-induced calcium transient crosses the egg and exocytosis occurs; a later increase in InsP₃, as calcium declines and the protein kinase C-dependent Na/H antiporter causes the cytoplasmic pH to increase. These results support suggestions that a calcium-induced hydrolysis of phosphatidylinositol bisphosphate occurs at fertilisation, that the production of diacylglycerol may be essential for exocytosis and that diacylglycerol production at fertilisation stimulates the Na/H antiporter. The increase in [³H]inositol polyphosphate as calcium declines indicates that this second messenger may have some function later in the cell cycle.

【 授权许可】

Unknown