Acetyl‐CoA carboxylase: a rapid novel assay procedure used in conjunction with the preparation of enzyme from maize leaves

FEBS Letters

Howard, Joy L.¹  Ridley, Stuart M.¹ 

[1] ICI Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire, England

关键词: Acetyl-CoA carboxylase;    Fluazifop;    Aryloxyphenoxypropionate;    Cyclohexanedione;    (Maize leaves);   

DOI  :  10.1016/0014-5793(90)80567-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acetyl-CoA carboxylase is known to be the site of action of two groups of grass-selective herbicides: the aryloxyphenoxypropionates and the cyclo-hexanediones. This enzyme was extracted from maize leaves, and a novel, highly sensitive assay system developed, utilising measurement of phosphate to estimate enzyme activity. This assay system was verified by using inhibition by fluazifop and avidin to compare it with the more widely used ¹⁴C assay. K _m values for acetyl-CoA were measured, and inhibition kinetics investigated.

【 授权许可】

Unknown