Granulosa cells as a source and target organ for tumor necrosis factor‐α

FEBS Letters

Meirom, Ruth¹  Shemesh, Mordechai¹  Mashiach, Shlomo²  Wollach, David³  Zolti, Mati²  Ben Rafael, Zion²  Shore, Laurence¹ 

[1] Departments of Hormone Research and Immunology, Kimron Veterinary Institute, Bet Dagan, Israel;Department of Obstetrics and Gynecology, Sheva Medical Center, Tel-Hashomer, Rehovot, Israel;Department of Virology, Weizmann Institute, Rehovot, Israel

关键词: Granulosa cell;    Tumor necrosis factor;    Follicle-stimulating hormone;    Ovarian follicle;    (Human;    Bovine);   

DOI  :  10.1016/0014-5793(90)80565-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tumor necrosis factor (TNF-α), a 17 kDa cytokine, is a product of activated macrophages which was recently shown to be produced by rat and bovine granulosa cells. In the present work, human granulosa cells derived from preovulatory follicles were used. It was demonstrated that human granulosa cells produce TNF-α (5–10 units/300 000 cells per 15 h). This production was increased by addition of follicle-stimulating hormone or by a combination of human chorionic gonadotrophin and CSF to the culture media. TNF was also found in bovine follicular fluid and the concentration was higher in the periovulatory than mid-cycle follicles. TNF-α was found to increase prostaglandin F-2α production by human granulosa cells (P < 0.001). We conclude that granulosa cells are both a source and target organ for TNF-α.

【 授权许可】

Unknown