FEBS Letters | |
Membrane‐bound F420H2‐dependent heterodisulfide reductase in methanogenic bacterium strain Göl and Methanolobus tindarius | |
Mahlmann, A.1  Blaut, M.1  Deppenmeier, U.1  Gottschalk, G.1  | |
[1]Institut für Mikrobiologie der Georg-August-Universität, Göttingen, FRG | |
关键词: F420; Heterodisulfide reductase; Methane formation; Membrane; Methanogenic bacteria; CoM-SH; 2-mercaptoethanesulfonate; DTNB; 5; 5'-dithio-bis(2-nitrobenzoic acid); F420; (N-L-lactyl-γ-L-glutamyl)-L-glutamic acid phosphodiester of 7; 8-didemethyl-8-hydroxy-5-deazariboflavin-5 '-phosphate; F420H2; reduced F420; MF; methano-furan; H4MPT; tetrahydromethanopterin; HTP-SH; 7-mercapto-heptanoylthreonine phosphate; Pipes; 1; 4-piperazinediethanesulfonic acid; Tricine; N-[2-hydroxy-1; 1-bis(hydroxymethyl)ethyl]-glycine; | |
DOI : 10.1016/0014-5793(90)80670-E | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Washed membrane or cytoplasmic fractions of the methanogenic bacterium strain Göl catalyzed the oxidation of coenzyme F420H2 with a variety of electron acceptors. The F420H2-oxidizing activity of the cytoplasmic fraction could be assigned to a NADP+:F420 oxidoreductase. The membrane fraction but not the cytoplasmic fraction catalyzed the oxidation of F420H2 with the concomitant reduction of the heterodisulfide of 2-mercapto-ethanesulfonate and 7-mercaptoheptanoylthreonine phosphate (CoM-S-S-HTP) at a rate of 100 protein according to the following equation: F420H2+CoM-S-S-HTP → F420 + CoM + HTP-SH. The activity depended linearly on the membrane protein up to a concentration of 60 The physiological electron acceptor CoM-S-S-HTP could not be replaced by the corresponding homodisulfides CoM-S-S-CoM and HTP-S-S-HTP or by NADP+. A membrane-bound F420H2-dependent CoM-S-S-HTP reductase was also detected in Methanolobus tindarius exhibiting a specific activity of 75 protein. The absence of a F420-dependent hydrogenase in this organism excludes the involvement of this enzyme in electron transfer from H420H2 to CoM-S-S-HTP.
【 授权许可】
Unknown
【 预 览 】
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