Cloning and sequencing of the gene encoding cytochrome c‐551 from Pseudomonas aeruginosa

FEBS Letters

Igarashi, Yasuo¹  Arai, Hiroyuki¹  Sanbongi, Yoshihiro¹  Kodama, Tohru¹ 

[1] Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan

关键词: Cytochrome c-551;    DNA sequence;    Signal peptide;    Nitrite reductase;    (Pseudomonas aeruginosa);   

DOI  :  10.1016/0014-5793(90)80669-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cytochrome c-551 gene from Pseudomonas aeruginosa was cloned by using two oligonucleotide probes, which had been synthesized based on the known primary structure of the protein. The restriction map of the cloned DNA and sequence analysis showed that the cytochrome c-551 gene is located 50 bp downstream of the nitrite reductase gene, which has recently been cloned and sequenced. DNA sequence analysis also indicated that cytochrome c-551 is synthesized in vivo as a precursor having an amino-terminal signal sequence consisting of 22 amino acid residues.

【 授权许可】

Unknown