Further studies on the human pancreatic binary complexes involving procarboxypeptidase A

FEBS Letters

Chapus, Catherine¹  Moulard, Maxime¹  Kerfelec, Brigitte¹  Michon, Thierry² 

[1] Centre de Biochimie et de Biologie Moléculaire du CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 9, France;Laboratoire de Biochimie et Technologie des Protéines I.N.R.A., Rue de la Géraudière, 44026 Nantes Cedex 03, France

关键词: Human pancreas;    Procarboxypeptidase;    Protease E;    Proelastase 2;    Protein complex;    DMMA;    2;    3 dimethylmaleic anhydride;    t-Boc Ala N-t-Boc-L-alanine p-nitrophenyl ester;    HPLA;    hippuryl-L-phenyllactic acid and HA;    hippuryl-L-arginine;    ProCPA;    procarboxypeptidase A;    proCPB;    procarboxypeptidase B;    CPA;    carboxypeptidase A;    CPB;    carboxypeptidase B;    Enzymes: (pro)carboxypeptidase A (EC 3.4.17.1);    (pro)carboxypeptidase B (EC 3.4.17.2);    pro(elastase);    (zymogen E) protease E (EC 3.4.21.36);    trypsin (EC 3.4.21.4);   

DOI  :  10.1016/0014-5793(90)80665-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In contrast to procarboxypeptidase B which has always been reported to be secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, depending on the species. Recent studies showed that, in the human pancreatic secretion, procarboxypeptidase A is mainly secreted as a 44 kDa protein involved in at least three different binary complexes. As previously reported, two of these complexes associated procarboxypeptidase A to either a glycosylated truncated protease E or zymogen E. In this paper, we identified proelastase 2 as the partner of procarboxypeptidase A in the third complex, thus reporting for the first time the occurrence of a proelastase 2/procarboxypeptidase A binary complex in vertebrates. Moreover, from N-terminal sequence analyses, the 44 kDa procarboxypeptidase A involved in these complexes was identified as being of the A₁ type. Only one type of procarboxypeptidase B, the B₁ type, has been detected in the analyzed pancreatic juices, thus emphasizing the previously observed genetic differences between individuals.

【 授权许可】

Unknown