Identification of a procarboxypeptidase A‐truncated protease E binary complex in human pancreatic juice

FEBS Letters

Chapus, Catherine²  Mallet, Bernard¹  Moulard, Maxime²  Kerfelec, Brigitte² 

[1] Laboratoire de Chimie Biologique (Faculté de Médecine), 27 B J. Moulin, 13385 Marseille Cedex 5, France;Centre de Biochimie et de Biologie Moléculaire du CNRS, Boîte Postale 71, F-13402, Marseille Cedex 9 France

关键词: Protease E;    Subunit III;    Binary complex;    Procarboxypeptidase A;    Truncated protease;    (Human);    proCPA-S6;    procarboxypeptidase A-S6 ternary complex;    proCPA;    procarboxypeptidase A;    HPE;    human protease E;    G32;    32 kDa protein associated with human procarboxypeptidase A;    Mes;    2[N-morpholino]ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(89)80712-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The characterization, in human pancreatic juice, of a binary complex associating procarboxypeptidase A with a 32 kDa inactive glycoprotein (G32) is reported in this paper. Free G32 was isolated after dissociation of the binary complex. N-terminal sequence analysis revealed a complete homology between this protein and human protease E (HPE 1), except for the two strongly hydrophobic N-terminal residues (Val-Val) which are missing in G32. This protein might be a truncated protease E highly analogous to the subunit III of the ruminant procarboxypeptidase A-S6 ternary complex. The analogy with bovine subunit III is further supported by interspecies reassociation experiments showing that bovine procarboxypeptidase A can specifically bind human G32.

【 授权许可】

Unknown