Polyamines improve Ca2+ transport system of the yeast mitochondria

FEBS Letters

Zvjagilskaya, Renata A.¹  Bazhenova, Elena N.¹  Votyakova, Tatyana V.¹ 

[1] A.N. Bach Institute of Biochemistry, Academy of Sciences of the USSR, Moscow 117071, USSR

关键词: Calcium ion;    Yeast mitochondria;    Spermine;    Spermidine;    (Endomyces magnusii);   

DOI  :  10.1016/0014-5793(90)80655-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Spermine at concentrations of 12–100 μM considerably activates the Ca²⁺ transport system of the Endomyces magnusii yeast mitochondria. As a result, in the presence of spermine the mitochondria are able to decrease extramitochondrial Ca²⁺ to the physiological level. At Ca²⁺ concentrations up to 200 μM, spermine enhances the initial rate of Ca²⁺ uptake (a half-maximal effect at 12 μM spermine). The Ca²⁺ concentrations required for half-maximal Ca²⁺ uptake rate to be achieved were 160 and 60 μM Ca²⁺ without and with spermine, respectively. Spermidine is shown to be less effective (a half-maximal effect at 50–100 μM spermidine). The polyamines do not change the parameters of energy coupling of mitochondria. The data obtained enabled the yeast mitochondria to be considered to take part in regulation of cytoplasmic and matrix Ca²⁺.

【 授权许可】

Unknown