Allicin, a naturally occurring antibiotic from garlic, specifically inhibits acetyl‐CoA synthetase

FEBS Letters

Lichtenthaler, Hartmut K.¹  Focke, Manfred¹  Feld, Andrea¹ 

[1] Botanisches Institut (Plant Physiology and Biochemistry) der Universität, Kaiserstr. 12, D-7500 Karlsruhe, FRG

关键词: Acetate kinase;    Acetyl-CoA synthetase;    Allicin;    Phosphotransacetylase;    (Garlic);   

DOI  :  10.1016/0014-5793(90)80647-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Allicin is shown to be a specific inhibitor of the acetyl-CoA synthetases from plants, yeast and mammals. The bacterial acetyl-CoA-forming system, consisting of acetate kinase and phosphotransacetylase, was inhibited too. Non-specific interaction with sulfhydryl-groups could be excluded in experiments with dithioerythritol and P-hydroxymercuribenzoate. Binding of allicin to the enzyme is non-covalent and reversible. [¹⁴C]-Acetate incorporation into fatty acids of isolated plastids was inhibited by allicin with an I₅₀ -value lower than 10 μM. Other enzymes of the fatty acid synthesis sequence were not affected, as was shown using precursors other than acetate.

【 授权许可】

Unknown