Partial processing of the neuropeptide Y precursor in transfected CHO cells

FEBS Letters

Wulff, Birgitte Schjellerup²  O'Hare, Mairead M.T.²  Theill, Lars E.¹  Boel, Esper³  Schwartz, Thue W.² 

[1] Institute of Molecular Biology, University ofAarhus, Århus, Denmark;Laboratory for Molecular Endocrinology, University Department of Chemistry, Rigshospitalet, Copenhagen, Denmark;Molecular Genetics, NOVO-Nordisk, Bagsværd, Denmark

关键词: Neuropeptide Y;    Peptide amidation;    Peptide processing;    Transfection;   

DOI  :  10.1016/0014-5793(90)80646-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activation of regulatory peptides by post-translational modification of their biosynthetic precursors is generally thought to occur only in neuroendocrine cells. We have selected clones of Chinese hamster ovary cells, a non-neuroendocrine cell line, which were transfected with a eukaryotic expression vector coding for the precursor for neuropeptide Y. Although the majority of the immunoreactive NPY was found in the form of pro-NPY, some degree of intracellular proteolytic processing of the precursor occurred in all clones. Part of the intracellular NPYimmunoreactivity was even correctly amidated. Extracellular degradation of pro-NPY in the tissue culture medium generated immunoreactivity which corresponded in size to NPY. It is concluded that precursor processing can occur in non-neuroendocrine cells both as a biological process within the cells and as apparent processing, degradation in the tissue culture medium.

【 授权许可】

Unknown