Members of the α‐amylase inhibitors family from wheat endosperm are major allergens associated with baker's asthma

FEBS Letters

Barber, Domingo¹  Lahoz, Carlos²  Armentia, Alicia²  Martin, Elena¹  Sánchez-Monge, Rosa³  Gómez, Luis³  de Andres, Belén²  Salced, Gabriel²  del Pozo, Victoria²  Hernández, Dolores¹  Palomino, Filar² 

[1] Departamento de Análisis, Alergia e Immunologia Abelló, MadridSpain;Sección de Alergia, Hospital Rio Ortega, Valladolid, Spain;Departamento de Bioquimica, ETS Ingenieros Agrónomes, MadridSpain

关键词: Allergen;    Baker's asthma;    α-Amylase inhibitor;    (wheat);    IEF;    isoelectrofocusing;    PVDF;    polyvinyldene difluoride;    RAST;    radioallergosorbent test;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    SGE;    starch gel electrophoresis;    WDAI-3;    wheat dimeric α-amylase inhibitor-3;   

DOI  :  10.1016/0014-5793(90)80642-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified the major antigens or IgE binding components from wheat flour. Thirty-five sera from patients with baker's asthma were used to analyze the reaction with wheat salt-soluble proteins. We found a 15 kDa SDS-PAGE band which reacted with all sera tested. Purified members of the α-amylase inhibitor family, which are the main components of the 15 kDa band, were recognized by specific IgE when tested with a pool of reactive sera. Immunodetection after two-dimensional electrophoretic fractionation of crude inhibitor preparations from wheat endosperms also detected several inhibitor subunits as major low-molecular-weight allergens.

【 授权许可】

Unknown