A barley flour inhibitor of insect α‐amylase is a major allergen associated with baker's asthma disease

FEBS Letters

Barber, Domingo⁴  Carpizo, José⁴  Armentia, Alicia³  Juan, Fernando⁴  Sánchez-Monge, Rosa²  Salcedo, Gabriel²  Gómez, Luis²  López-Otín, Carlos¹ 

[1] Departamento de Bioquímica, Facultad de Medicina, Universidad de Oviedo, Oviedo, Spain;Departamento de Bioquímica, ETS Ingenieros Agrónomos, Madrid, Spain;Sección de Alergia, Hospital Rio Ortega, Valladolid Spain;Departamento de Análisis, Alergía e Inmunologia Abelló, ETS Ingenieros Agrónomos, Madrid, Spain

关键词: Amylase inhibitor;    α-;    Allergen;    Baker's asthma;    (Barley);    IEF;    isoelectrofocusing;    RAST;    radioallergosorbent test;    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    SGE;    starch gel electrophoresis;    RP-HPLC;    reversed-phase high-performance liquid chromatography;   

DOI  :  10.1016/0014-5793(89)80444-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A barley salt-soluble protein of 14.5 kDa, which inhibits the α-amylase from the insect Tenebrio molitor, has been identified as a major IgE-binding component of sera from baker's asthma patients. The N-terminal amino acid sequence of this protein indicates that it is a member of a previously described family of α-amylase/trypsin inhibitors.

【 授权许可】

Unknown