Mitogen‐induced oscillations of membrane potential and Ca2+ in human fibroblasts

FEBS Letters

Peres, Antonio¹  Giovannardi, Stefano¹ 

[1] Dip. di Fisiologia e Biochimica Gen. dell' Universita' di Milano, Via Celoria 26, 20133 Milano, Italy

关键词: Mitogen;    Cytoplasmic Ca2+;    Hyperpolarization;    Oscillation;    K+ channel;    (Fibroblast);   

DOI  :  10.1016/0014-5793(90)80630-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the whole-cell technique, we have measured recurring hyperpolarizations induced by fetal calf serum and bradykinin in human fibroblasts. By coupling fura-2 microfluorimetry to electrophysiology, we have also measured directly cytosolic Ca²⁺ and found that Ca²⁺ oscillations occur in synchrony with membrane currents. Mitogen stimulation of cells in which intracellular K⁺ had been replaced with Cs⁺ resulted in the abolishment of the outward current. We conclude then that the mitogen-induced recurring hyperpolarizations in human fibroblasts are due to the opening of Ca²⁺-activated K⁺ channels.

【 授权许可】

Unknown