Is a four‐state model sufficient to describe actomyosin ATPase?

FEBS Letters

Travers, Franck¹  Barman, Thomas¹  Tesi, Chiara¹ 

[1] INSERM U128, CNRS, BP 5051, 34033, Montpellier, France

关键词: Actomyosin ATPase;    Four-state;    Six-state;    Pi burst;    transient;   

DOI  :  10.1016/0014-5793(90)80110-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four- [(1984), J. Biol. Chem. 259, 11908] and six- [(1985) Science 227, 999] state models have been proposed for actomyosin ATPase. A key experiment in deciding between these is whether or not there is a transient P_i burst at high actin. In the first, the cleavage and release of products rates are similar and the P_i burst is low; in the second, there are additional product complexes and the P_i burst is large. We reinvestigated the problem by carrying out burst experiments under the conditions in [(1985) Science 227, 999]. Since we find that the P_i burst at high actin is low, we conclude that the four-state model is sufficient to describe actomyosin ATPase.

【 授权许可】

Unknown