Video enhanced imaging of the fluorescent Na+ probe SBFI indicates that colonic crypts absorb fluid by generating a hypertonic interstitial fluid

FEBS Letters

Pedley, K.C.¹  Naftalin, R.J.¹ 

[1] Department of Physiology, King's College London; Strand and Kensington Campuses, London WC2 2LS, England

关键词: Fluorescent imaging;    Sodium-ion;    Colon;    Absorption;   

DOI  :  10.1016/0014-5793(90)80100-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Extracellular accumulation of Na⁷⁺ detected by video-enhanced microscopic imaging of the impenneant fluorescent probe SBFI[1,2] confirms the view that colonic crypts produce a hypertonic absorbate ca 1000 mOsm·kg⁻¹, thereby generating a large osmotic pressure across the crypt wall. This creates a high fluid tension within the crypt lumen, sufficient to dehydrate faeces [3,4]. When bathed in Tyrode the SBFI·Na⁺ fluorescence indicates a [Na⁺) ca 750 mM within the interstitial space of metabolizing rat descending colon. There is no evidence of interstitial Na⁺ accumulation in octanol (2 mM) or in rabbit colon incubated with 1.0 mM ouabain and no evidence of Na⁺ secretion via the crypt lumen during absorption [5].

【 授权许可】

Unknown