8 nm nanodiamonds as markers for 2 photon excited luminescent microscopy

5th International Symposium on Coherent Optical Radiation of Semiconductor Compounds and Structures

Kharin, A.^1 ; Rogov, A.^2 ; Geloen, A.^3 ; Lysenko, V.^4 ; Bonacina, L.^2

National Research Nuclear University MEPhI, 31 Kashirskoye shosse, Moscow

115409, Russia^1

GAP-Biophotonics, University of Geneva, ch. de Pinchat 422, Geneva

CH-1211, Switzerland^2

University of Lyon, CarMeN Laboratory, UMR INSERM 1060, INSA de Lyon, University of Lyon, France^3

University of Lyon, Nanotechnology Institute of Lyon (INL) UMR 5270, CNRS, INSA Lyon, Villeurbanne

F-69621, France^4

关键词: Average diameter;    Fluorescent imaging;    Hydrodynamic Radius;    Low toxicity;    Luminescent microscopies;    Luminescent property;    Stable suspensions;    Two-photon excitations;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/740/1/012010/pdf
DOI  :  10.1088/1742-6596/740/1/012010

来源: IOP

【 摘 要 】

Structural and luminescent properties of stable suspensions of fluorescent nanodiamonds were investigated. Measurement of the effective hydrodynamic radius yields particles less than 30 nm diameter, while the TEM measurements made on the same particles shows average diameter about 8 nm. It was found that NDs have relatively low toxicity. Upon incubation, 3T3-L1 cells spontaneously take up nanodiamonds that uniformly distribute in cells cytoplasm. The possibility of fluorescent imaging using both single ore two-photon excitation was shown.