Interactions between nitrogen oxide‐containing compounds and peripheral benzodiazepine receptors

FEBS Letters

Bolger, Gordon T.¹  Weissman, Ben A.²  Chiang, Peter K.² 

[1] Division of Basic Medical Sciences Memorial University of Newfoundland, St. Johns, Newfoundland, H7S2G5 Canada;Department of Applied Biochemistry, Walter Reed Army Institute of Research, Washington, DC 20307-5100, USA

关键词: Benzodiazepine receptor;    peripheral;    Sodium nitroprusside;    (Rat atrium;    Guinea pig heart);   

DOI  :  10.1016/0014-5793(90)80095-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nitrogen oxide-containing compounds displaced the peripheral benzodiazepine ligand [³H]Ro5-4864 from guinea pig membrane preparations. Sodium nitroprusside (SNP) was the most potent (IC ₅₀ = 5.61 ± 1.72 × 10⁻⁵ M). Moreover, its ability to bind to these receptors showed marked tissue variability (heart > kidney ⪢ cerebral cortex). When tested on rat atrium, SNP by itself had no effect on basal inotropy or the increase in inotropy induced by (−)-S-BAY K 8644. In contrast, Ro5-4864 potentiated the marked increase in inotropy induced by (−)-S-Bay K 8644, and SNP completely abolished the potentiation of inotropy observed with Ro5-4864. Since peripheral benzodiazepine receptors are associated with calcium mobilization in the heart, these findings may indicate that some of the clinical effects of nitric oxide-generating drugs could be mediated by these receptors.

【 授权许可】

Unknown