Structure of the gene encoding concanavalin A from Canavalia gladiata and its expression in Escherichia coli cells

FEBS Letters

Yamauchi, Daisuke¹  Minamikawa, Takao¹ 

[1] Department of Biology, Tokyo Metropolitan University, Fukazawa, Setagaya-ku, Tokyo, 158 Japan

关键词: Concanavalin A;    Gene structure;    Legume lectin;    Carbohydrate binding activity;    Processing;    Peptide ligation;    (Canavalia gladiata;    Escherichia coli);    Con A;    concanavalin A;    PAGE;    polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(90)80083-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned and sequenced the gene encoding concanavalin A (Con A) from Canavalia gladiata. The sequence covers the whole transcribed region as well as the 5'- and 3'-untranscribed sequences. The coding sequence lacks introns. Con A expressed in Escherichia coli cells was purified by Sephadex G-50 affinity chromatography. The precursor of Con A expressed in E. coli undergoes a peptide cleavage and ligation in the same way as that synthesized during seed maturation.

【 授权许可】

Unknown