Cloning and sequencing of winged bean (Psophocarpus tetragonolobus) basic agglutinin (WBA I): presence of second glycosylation site and its implications in quaternary structure

FEBS Letters

Srinivas, V.R.¹  Sharma, Vivek¹  Surolia, Avadhesha¹ 

[1] Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India

关键词: Primary structure;    Legume lectin;    PCR cloning;    Sequence homology;    Carbohydrate-binding site;    N-linked glycosylation site;   

DOI  :  10.1016/0014-5793(96)00613-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report cloning of the DNA encoding winged bean basic agglutinin (WBA I). Using oligonucleotide primers corresponding to N- and C-termini of the mature lectin, the complete coding sequence for WBA I could be amplified from genomic DNA. DNA sequence determination by the chain termination method revealed the absence of any intervening sequences in the gene. The DNA deduced amino acid sequence of WBA I displayed some differences with its primary structure established previously by chemical means. Comparison of the sequence of WBA I with that of other legume lectins highlighted several interesting features, including the existence of the largest specificity determining loop which might account for its oligosaccharide-binding specificity and the presence of an additional N-glycosylation site. These data also throw some light on the relationship between the primary structure of the protein and its probable mode of dimerization.

【 授权许可】

Unknown