FEBS Letters | |
High molecular weight forms of basic fibroblast growth factor recognized by a new anti‐bFGF antibody | |
Zachmann, Karolin1  Westermann, Reiner1  Grothe, Claudia1  Unsicker, Klaus1  | |
[1]Department of Anatomy and Cell Biology, Philipps-University of Marburg, Robert-Koch-Str. 6, D-3550 Marburg, FRG | |
关键词: Fibroblast growth factor; basic; Ovary; Adrenal; Pituitary; Antibody; | |
DOI : 10.1016/0014-5793(90)80059-R | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
An antibody against basic fibroblast growth factor (bFGF) was raised using purified bovine pituitary bFGF. Western blot analysis revealed immunoreactive bands at 18, 24, 30–33 and 46 kDa in inununoaffinity purified extracts of pituitary and adrenal gland using this antibody. A similar staining pattern was obtained with ovary extracts with the exception of the missing 18 kDa band. A second anti-bFGF antibody raised against a synthetic peptide comprising the 24 N-terminal amino acids of bFGF reacted with the 18 kDa and the 46 kDa band of immunoaffinity purified ovary and adrenal gland extracts.
【 授权许可】
Unknown
【 预 览 】
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