Photolabile μ‐conotoxins with a chromogenic phenyldiazirine

FEBS Letters

Kanaoka, Yuichi²  Satake, Mei¹  Abe, Teruo¹  Hatanaka, Yasumaru²  Yoshida, Eiichi²  Nakayama, Hitoshi² 

[1] Department of Neurochemistry, Brain Research Institute, Niigata University, Niigata 951, Japan;Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: Diazirine;    μ-Conotoxin;    Photoaffinity labeling;    Sodium channel;   

DOI  :  10.1016/0014-5793(90)80057-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three photoreactive derivatives of μ-conotoxin G IIIA have been prepared as photoaffinity labeling reagents for muscle-type sodium channels. The reagents competitively inhibited the binding of saxitoxin to the eel sodium channel with K _i values of 11–18 nM. The introduced chromogenic phenyldiazirine group on the toxin was photolyzed efficiently, and spectroscopic properties of the reagents demonstrated that irradiation and detection can be performed in a spectral region where the absorptions due to most of biological macromolecules are negligible.

【 授权许可】

Unknown