A new class of antivirals: antisense oligonucleotides combined with a hydrophobic substituent effectively inhibit influenza virus reproduction and synthesis of virus‐specific proteins in MDCK cells

FEBS Letters

Melik-Nubarov, Nikolai S.¹  Krivonos, Alexander V.¹  Ovcharenko, Alexander V.¹  Kiselev, Vsevolod I.¹  Kabanov, Alexander V.¹  Vinogradov, Sergei V.¹  Severin, Eugenii S.¹ 

[1]Research Center of Molecular Diagnostics, USSR Ministry of Health, Simpheropolsky blvd8, Moscow 113149, USSR

关键词: Antisense;    Oligonucleotide;    Influenza;    Virus;    Inhibition;    Translation;    Aerosol OT;    sodium bis(2-ethylhexyl)sulfosuccinate;    Hepes;    N(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid sodium salt;    Mes;    2-(N-morpholino)ethanesulfonic acid;    PFU;    plaque forming units;   

DOI  :  10.1016/0014-5793(90)80039-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To enhance the penetration of oligonucleotide (‘oligo’) into cells, the oligo was combined with the hydrophobic undecyl residue. Using the ‘DNA-synthesator’, we synthesized oligo, complementary to the loop-forming site of the RNA, encoding polymerase 3 of the influenza virus (type A), and combined it with the undecyl residue added to the 5' terminal phosphate group. It was found that the modified oligo effectively suppresses the influenza A/PR8/34 (H1N1) virus reproduction and inhibits the synthesis of virus-specific proteins in MDCK cells. Under the same conditions, the non-modified antisense oligo and modified nonsense oligo did not affect the virus development.

【 授权许可】

Unknown