Fluorescence properties of the Ca2+Mg2+‐ATPase protein of sarcoplasmic reticulum labeled with 7‐chloro‐4‐nitrobenzo‐2‐oxa‐1,3‐ diazole

FEBS Letters

Bailin, Gary¹  Huang, John R.¹ 

[1]Department of Biochemistry, UMDNJ-School of Osteopathic Medicine, 675 Hoes Lane, Piscataway, NJ 08854-5635, USA

关键词: Sarcoplasmic reticulum;    Skeletal muscle;    ATPase;    Fluorescence labeling;   

DOI  :  10.1016/0014-5793(90)80021-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fluorescence intensity of the Ca²⁺,Mg²⁺-ATPase protein of rabbit skeletal sarcoplasmic reticulum that incorporated about 2 mol of 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (NBD-Cl) was enhanced at high MgATP concentrations with or without 50 μM calcium. The observed enhancement indicates that the fluorophore, NBD-Cl, can detect conformational changes in the ATPase protein.

【 授权许可】

Unknown