Phosphatidylinositol hydrolysis by human plasma phospholipase D

FEBS Letters

Mollinedo, Faustino¹  Balsinde, Jesus¹ 

[1] Centro de Investigaciones Biológicas (CSIC), Madrid, Spain

关键词: Phosphatidylinositol;    Phospholipase D;    Human plasma;   

DOI  :  10.1016/0014-5793(90)80017-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A phospholipase D activity able to hydrolyze phosphatidylinositol has previously been described in the cytosol of human neutrophils. The experiments reported here demonstrate that this phosphatidylinositol-hydrolyzing phospholipase D activity is also present in human plasma. This activity was assessed by free inositol release from phosphatidylinositol substrate, by phosphatidate formation and by phosphatidylethanol formation through its capacity of catalyzing a transphosphatidylation reaction. This plasma enzyme activity shows an optimum pH of 8.0 and is inhibited by EGTA.

【 授权许可】

Unknown