A single point mutation confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II

FEBS Letters

Suzuki, Harukazu¹  Stühmer, Walter²  Noda, Masaharu¹  Numa, Shosaku¹ 

[1] Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto 606, Japan;Max-Planck-Institut für biophysikalische Chemie, D-3400 Göttingen, FRG

关键词: Site-directed mutagenesis;    cDNA expression;    Sodium channel;    Tetrodotoxin;    Saxitoxin;    (Xenopus oocyte);    TTX;    tetrodotoxin;    STX;    saxitoxin;    TMO;    trimethyloxonium;   

DOI  :  10.1016/0014-5793(89)81531-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A single point mutation of the rat sodium channel II reduces its sensitivity to tetrodotoxin and saxitoxin by more than three orders of magnitude. The mutation replaces glutamic acid 387 with a glutamine and has only slight effects on the macroscopic current properties, as measured under voltage-clamp in Xenopus oocytes injected with the corresponding cDNA-derived mRNA.

【 授权许可】

Unknown