Functional expression of the rat heart I Na+ channel isoform Demonstration of properties characteristic of native cardiac Na+ channels

FEBS Letters

Cribbs, Leanne L.¹  Rogart, Richard B.¹  Fozzard, Harry A.¹  Satin, Jonathan¹ 

[1] Department of Medicine, Section of Cardiology, University of Chicago, 5841 S. Maryland Avenue, Chicago, IL 60637, USA

关键词: Na+ channel;    cDNA expression;    Xenopus oocyte;    Na+ current;    Tetrodotoxin;    Saxitoxin;    TTX;    tetrodotoxin;    STX;    saxitoxin;    I Na;    sodium current;    TTX-S;    TTX-sensitive;    TTX-R;    TTX-resistant;    RH-I;    rat heart I Na + channel;   

DOI  :  10.1016/0014-5793(90)81470-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the expression of functional Na⁺ channel in Xenopus oocytes injected with cRNA transcribed from the rat heart 1 cDNA clone. The expressed rat heart 1 Na⁺ currents show kinetic properties and resistance to tetrodotoxin and saxitoxin which are characteristic of native cardiac Na⁺ currents. The primary amino acid sequence of the rat heart 1 α-subunit is therefore sufficient for expression of of tetrodotoxin resistance, and the rat heart 1 clone is likely to account for the tetrodotoxin-resistant phenotype of cardiac and denervated skeletal muscle.

【 授权许可】

Unknown