Platelet‐activating factor (PAF) acts as an intercellular messenger in the changes of cytosolic free Ca2+ in human neutrophils induced by opsonized particles

FEBS Letters

Koenderman, Leo¹  Roos, Dirk¹  Tool, Anton T.J.¹  Verhoeven, Arthur J.¹ 

[1] Central Laboratory of the Netherlands Red Cross, Blood Transfusion Service, University of Amsterdam, Amsterdam, The Netherlands

关键词: [Ca2+];    cytosolic free;    Human neutrophil;    Opsonized particle;    Platelet-activating factor;    Intercellular messenger;   

DOI  :  10.1016/0014-5793(89)81530-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Additon of opsonized particles to human neutrophils in suspension leads to a biphasic elevation in the cytosolic free Ca²⁺ concentration ([Ca²⁺]_i). The rise in [Ca²⁺]_i, during the second phase (> 3 min) is pronounced (about 400 nM), in contrast to the rise during the first phase, which is relatively small (< 100 nM). The second and large rise in [Ca²⁺]_i is brought about by messenger(s) released from the cell after addition of opsonized particles. This second rise in [Ca²⁺]_i, is not observed in the presence of the platelet-activating factor (PAF) antagonist WEB 2086, indicating that PAF can act as an intercellular messenger affecting Ca²⁺ homeostasis in human neutrophils.

【 授权许可】

Unknown