Inhibitors of arachidonic acid metabolism eliminate the increase in cytosolic free calcium induced by the mitogen concanavalin A in rat thymocytes

FEBS Letters

Pulido, H.Arias¹  Gukovskaya, A.S.¹  Zinchenko, V.P.¹  Evtodienko, Yu.V.¹ 

[1] Institute of Biological Physics, USSR Academy of Sciences, Pushchino, Moscow Region 142292, USSR

关键词: Arachidonic acid;    Ca2+;    cytosolic free;    Concanavalin A;    (Rat thymocyte);    AA;    arachidonic acid;    NDGA;    nordihydroguaiaretic acid;    Con A;    concanavalin A;    BCECF;    2′;    7′-biscarboxyethyl-5(6)-carboxyfluoresceine;    [Ca2+]i;    cytosolic free Ca2+ concentration;    pHi;    cytosolic pH;    PLA2;    phospholipase A2;   

DOI  :  10.1016/0014-5793(89)80583-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using inhibitors of arachidonic acid (AA) metabolism, the possible involvement of AA products in the generation of [Ca²⁺]_i and the pH_i rise induced by the mitogen concanavalin A (Con A) in rat thymocytes has been studied. The lipoxygenase inhibitor nordihydroguaiaretic acid (NDGA, 10 μM) and the phospholipase A₂ inhibitor bromophenacyl bromide (10 μM) eliminated the [Ca²⁺]_i signal induced by Con A; the cyclooxygenase blocker indomethacin also inhibited it. However, neither NDGA nor indomethacin suppressed the pH_i rise stimulated by Con A. Exogenous AA induced an increase in [Ca²⁺]_i but not in the pH_i. These results indicate that AA metabolites, probably of the lipoxygenase pathway, take part in the generation of the [Ca²⁺]_i response to the mitogen. In contrast, they appear not to be involved in the pH_i rise evoked by Con A.

【 授权许可】

Unknown