Expression of a deletion mutant of the prosegment of human prorenin in Chinese hamster ovary cells

FEBS Letters

Hori, Hitoshi²  Nagahama, Masami²  Murakami, Kazuo²  Nakayama, Kazuhisa¹ 

[1] Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305, Japan;Institute of Applied Biochemistry, University of Tsukuba, Tsukuba, Ibaraki 305, Japan

关键词: Renin;    Gene expression;    Protein folding;    (Chinese hamster ovary cell);    CHO cells;    Chinese hamster ovary cells;    AI;    angiotensin I;   

DOI  :  10.1016/0014-5793(89)81528-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression plasmids encoding native human preporenin and a mutant deleted in its entire prosegment were transfected into Chinese hamster ovary cells. The cells transfected with the expression plasmid of native preporenin secreted exclusively inactive prorenin, while the cells transfected with the mutant secreted the active enzyme. The secreted amount of renin from the latter cells was much lower than that of prorenin from the former ones, although these two enzymes had little difference in specific activity after trypsin activation. These results suggest that the prosegment plays an important role in the secretory process of renin, although the fully active enzyme can be formed in its absence.

【 授权许可】

Unknown