Role of N‐linked oligosaccharides attached to human renin expressed in COS cells

FEBS Letters

Hori, Hitoshi¹  Murakami, Kazuo¹  Ishizuka, Yasuyuki¹  Yamauchi, Takeshi¹  Yoshino, Teruhiko¹ 

[1] Institute of Applied Biochemistry, University of Tsukuba, Ibaraki 305, Japan

关键词: Renin;    N-linked oligosaccharide;    Site-directed mutagenesis;    Gene expression;    (COS cell);    SDS-PAGE;    SDS-polyacrylamide gel electrophoresis;    DMEM;    Dulbecco's modified Eagle medium;    AI;    angiotensin I;   

DOI  :  10.1016/0014-5793(88)80777-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

One or both of two putative N-glycosylation sites (at asparagine-5 and -75) of human renin was eliminated by amino acid replacement of the asparagine residue with an alanine residue using site-directed mutagenesis. The three glycosylation-deficient renins (Asn-5, Asn-75, Asn-S and -75 mutants) were expressed in COS cells and secreted into the conditioned media. The secreted amounts of the three mutants were different from one another, although the mutant and wildtype renins had practically the same specific activity. An Asn-5 and -75 mutant which did not contain any glycosylation sites was unstable in the medium, suggesting that the N-linked oligosaccharides play an important role in stabilization of human renin.

【 授权许可】

Unknown