Nuclear envelope assembly around sperm chromatin in cell‐free preparations from Drosophila embryos

FEBS Letters

Ulitzur, Nirit¹  Gruenbaum, Yosef¹ 

[1] Department of Genetics, The Life Sciences Inst., The Hebrew University of Jerusalem, Jerusalem 91904, Israel

关键词: Chromatin;    Nuclear envelope;    Lamin;    Chicken sperm;    (Drosophila melanogaster);   

DOI  :  10.1016/0014-5793(89)81507-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chicken sperm chromatin initiated an assembly of interphase-like nuclei in a cell-free cytoplasmic preparation from 1–6 h old Drosophila melanogaster embryos. The formation of these interphase-like nuclei from the condensed sperm chromatin happened in a series of distinct steps. Anti-Drosophila lamin monoclonal antibody stained the assembled nuclei in a pattern indistinguishable from normal Drosophila nuclei. This assembly process required an ATP regenerating system and could be blocked by the addition of novobiocin into the cell-free extract.

【 授权许可】

Unknown