A synthetic peptide substrate specific for casein kinase‐1

FEBS Letters

Goris, Jozef¹  Merlevede, Wilfried¹  Vandenheede, Jackie R.¹  Pinna, Lorenzo A.²  Agostinis, Patrizia¹  Marin, Oriano²  Meggio, Flavio² 

[1] Afdeling Biochemie, Faculteit Geneeskunde, Katholieke Universiteit te Leuven, Belgium;Dipartimento di Chimica Biologica e Centro Studi Fisiologia Mitocondriale del Centro Nazionale delle Ricerche, Universitá di Padova, Italy

关键词: Casein kinase-1;    Peptide phosphorylation;    Protein kinase specificity;   

DOI  :  10.1016/0014-5793(89)81498-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The synthetic peptide, Asp-Asp-Asp-Glu-Glu-Ser-Ile-Thr-Arg-Arg, derived from the phosphorylation site of casein kinase-1 (CK-1) in β-casein A², is readily phosphorylated by CK-1, but not by casein kinase-2 (CK-2), cyclic AMP-dependent protein kinase, protein kinsae C, phosphorylase kinase and protein kinase F_a. Phosphorylation by CK-1 occurs only at Ser-6, Thr-8 being unaffected. The K _m for the peptide is higher (1 mM) than for β-casein A² (40 μM), while the V _maxis quite comparable. This is the first synthetic peptide substrate for CK-1 described so far, and can be used for the rapid and specific estimation of CK-1 activity in crude extracts.

【 授权许可】

Unknown