Substrate specificity determinants of the checkpoint protein kinase Chk1

FEBS Letters

Hutchins, James R.A.¹  Hughes, Mike¹  Clarke, Paul R.¹ 

[1] Biomedical Research Centre, University of Dundee, Level 5, Ninewells Hospital and Medical School, Dundee DD1 9SY, UK

关键词: Cdc25;    Chk1;    Cell cycle checkpoint;    Protein kinase specificity;   

DOI  :  10.1016/S0014-5793(99)01763-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Chk1 protein kinase plays a critical role in a DNA damage checkpoint pathway conserved between fission yeast and animals. We have developed a quantitative assay for Chk1 activity, using a peptide derived from a region of Xenopus Cdc25C containing Ser-287, a known target of Chk1. Variants of this peptide were used to determine the residues involved in substrate recognition by Chk1, revealing the phosphorylation motif Φ-X-β-X-X-(S/T)*, where * indicates the phosphorylated residue, Φ is a hydrophobic residue (M>I>L>V), β is a basic residue (R>K) and X is any amino acid. This motif suggests that Chk1 is a member of a group of stress-response protein kinases which phosphorylate target proteins with related specificities.

【 授权许可】

Unknown