In vitro translation of halobacterial mRNA

FEBS Letters

Oesterhelt, D.¹  Gropp, R.¹ 

[1] Max-Planck-Institutfür Biochemie, D-8033 Martinsried, FRG

关键词: In vitro translation;    Bacteriorhodopsion;    Archaebacterium (Halobacterium halobium);    SDS;    sodium dodecyl sulfate;    TCA;    trichloroacetic acid;    PPO;    2;    5-diphenyloxazole;    BO;    bacterio-opsin;    BR;    bacteriorhodopsin;    bop;    bacterio-opsin gene;    hop;    halorhodopsin gene;    brp;    bacteriorhodopsin-related protein gene;   

DOI  :  10.1016/0014-5793(89)81481-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A system for in vitro translation of halobacterial mRNA is described. It is composed of 70S ribosomes, a nuclease-treated S100 supernatant, yeast tRNA and halobacterial mRNA. Specific expression of proteins could be demonstrated by translation of messages from halophage ϑ1-infected cells. However, under no conditions could a pure bacterio-opsin message be observed to be active in translation. This points to a more complex translational property of bacterio-opsin and possibly other membrane proteins in archaebacteria.

【 授权许可】

Unknown