Crystallization and preliminary X‐ray crystallographic study of the quinoprotein methanol dehydrogenase from bacterium W3A1

FEBS Letters

Hao, Zhi-Ping³  Xia, Zong-Xiang³  Mathews, F.Scott¹  Davidson, Victor L.² 

[1] Department of Cell Biology and Physiology, Washington University School of Medicine, St. Louis, MO 63110, USA;Department of Biochemistry, The University of Mississippi Medical Center, Jackson, MS 39216-4505, USA;Shanghai Institute of Organic Chemistry, Academia Sinica, Shanghai, PR, China

关键词: Crystallization;    Methanol dehydrogenase;    Pyrroloquinoline quinone;    Quinoprotein;   

DOI  :  10.1016/0014-5793(89)81644-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methanol dehydrogenase from bacterium W3A1 has been crystallized by the macroseeding method to give single crystals suitable for three-dimensional structural study at resolution greater than 3 Å. The crystals belong to the space group P2₁ and have unit cell dimensions a = 124.13 Å, b = 62.87 Å, c = 84.71 Å, and β = 92.89°. There is one dimeric molecule of 114600 Da per asymmetric unit.

【 授权许可】

Unknown