期刊论文详细信息
FEBS Letters
NAD‐dependent, PQQ‐containing methanol dehydrogenase: a bacterial dehydrogenase in a multienzyme complex
Berkhout, M.P.J.1  Duine, J.A.1  Frank, J.1 
[1] Laboratory of Biochemistry, Delft University of Technology, Julianalaan 67, 2628 BC Delft, The Netherlands
关键词: Methanol dehydrogenase;    NAD-dependent aldehyde dehydrogenase;    NADH dehydrogenase;    Multienzyme complex;    Pyrroloquinoline quinone;    Nocardia sp. 239;    PQQ;    pyrroloquinoline quinone;    DCIP;    2;    6-dichlorophenolindophenol;   
DOI  :  10.1016/0014-5793(84)80249-5
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

Cell-free extracts of methanol-grown Nocardia sp. 239 only show significant dye-linked methanol-oxidizing activity when NAD+ is added to the assay mixture. This activity resides in a multienzyme complex which could be resolved into 3 components, namely the methanol dehydrogenase, NAD-dependent aldehyde dehydrogenase and NADH dehydrogenase. In its dissociated form, the methanol dehydrogenase no longer shows dye reduction and although rises in the absorbance values around 340 nm are seen on addition of methanol plus NAD+ to the enzyme, this is not due to NADH production. However, dye reduction (NAD dependent) could be restored on incubating methanol dehydrogenase with the corresponding NADH dehydrogenase, obtained from the enzyme complex. It is concluded that this novel methanol dehydrogenase transfers the reducing equivalents, derived from methanol, directly to its associated NADH dehydrogenase via a mechanism in which NAD+ and PQQ are involved.

【 授权许可】

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